Bone marrow mesenchymal stem cells protect myocardial function in acute myocardial infarction through a paracrine mechanism

doi:10.3969/j.issn.2095-4344.2014.23.008

Abstract

Abstract:

BACKGROUND: Adult cardiomyocytes show no regenerative ability, and cell therapy for myocardial regeneration and repair may improve myocardial ischemic injury function.

OBJECTIVE: To confirm the effect and reveal the mechanism of bone marrow mesenchymal stem cells (BMSCs) on acute myocardial infarction (AMI).

METHODS: BMSCs were isolated, cultured from bone marrow of Sprague-Dawley rats using density gradient centrifugation. AMI models were produced in 20 rats by ligating the left anterior descending (LAD) coronary artery, and randomly divided into model group and BMSCs group. In the BMSCs group, cells were subsequently injected with a sterile microinjection via the tail vein.

RESULTS AND CONCLUSION: Six months postoperatively, the cardiac function was improved, the vessel density was increased, the percentage of apoptotic cells was decreased in the BMSCs group than that in the model group; the expression levels of inflammatory factors, including vascular endothelial growth factor, von Willebrand factor, transforming growth factor 3β, and interleukin-1β mRNA were significantly improved in the BMSCs group than that in the model group. These results showed that BMSCs can protect the myocardium from AMI by regulating the secretion of inflammatory cytokines and angiogenic factors.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: myocardium, myocardial infarction, mesenchymal stem cells, mesenchymal stem cell transplantation, intercellular signaling peptides and proteins

CLC Number:

R394.2

Zou Song-ping, Wang Yu, Li Chun-yu, Fu Xue-dan, Liu Yan-li. Bone marrow mesenchymal stem cells protect myocardial function in acute myocardial infarction through a paracrine mechanism[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(23): 3653-3659.

Figures/Tables 2

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